Sequence requirement for peptide recognition by rat brain p21ras protein farnesyltransferase.

Sequence requirement for peptide recognition by rat brain p21ras protein farnesyltransferase.

We tested 42 tetrapeptides for their ability to bind to the rat brain p21ras protein farnesyltransferase as estimated by their ability to compete with p21Ha-ras in a farnesyltransfer assay. Peptides with the highest affinity had the structure Cys-A1-A2-X, where positions A1 and A2 are occupied by aliphatic amino acids and position X is occupied by a COOH-terminal methionine, serine, or phenylal...

Partial characterization of p21ras farnesyltransferase present in human placental tissue.

Protein Sequence Grouping by Peptide Word Motifs

Methods for collecting related segments from the protein sequence database using strongly conserved peptide words as well as sequence homology was applied to the problem of reconstruction of PROSITE catalog [1] from the sequence database. In many case our results were well consistent with PROSITE although some additional relationships were also found.

Time-dependent inhibition of protein farnesyltransferase by a benzodiazepine peptide mimetic.

Protein farnesyltransferase (FTase) and protein geranylgeranyltransferase-I (GGTase-I) catalyze the prenylation of proteins with a carboxy-terminal tetrapeptide sequence called a CaaX box, where C refers to cysteine, "a" refers to an aliphatic residue, and X typically refers to methionine, serine, or glutamine (FTase), or to leucine (GGTase-I). Marsters and co-workers [(1994) Bioorg. Med. Chem....

Neuroprotection against ischaemic brain injury by a GluR6-9c peptide containing the TAT protein transduction sequence.

It is well documented that N-methyl-D-aspartate and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptors play a pivotal role in ischaemic brain injury. Recent studies have shown that kainate (KA) receptors are involved in neuronal cell death induced by seizure, which is mediated by the GluR6*PSD-95*MLK3 signalling module and subsequent c-Jun N-terminal kinase (JNK) activation. Here w...